The writing of the Handbook of Paediatric AIDS in Africa by over 30 clinicians and social scientists on the African continent was completed in 2004. The writing workshops were funded by USAID/EA with passionate and enthusiastic support by Mary Pat Kieffer, who was then in USAID/EA. The book was launched in 2005 in Dar es Salaam Tanzania by Jeffrey Ashley, then in-charge of HIV programs at USAID/East Africa.  This handbook became a popular resource for training health workers on paediatric HIV/AIDS in Africa.

During this period, focus for the network was mainly on strengthening technical capacity for paediatric HIV care and treatment in various countries in the region through training of health care service providers.

Paediatric HIV training programs were initiated in Kenya, Tanzania, Rwanda, Zambia, Namibia, and later Burundi and the DRC where over 800 health care workers were trained.  Nine countries (Kenya, Tanzania, Rwanda, Zambia, Namibia, Uganda, Ghana, Burundi and Lesotho) adapted and/or used the ANECCA paediatric HIV care and treatment curriculum into /as respective national training curricula.

In 2007, a Regional Workshop to Support Scale Up of Paediatric Services in West and Central African Countries was held in Dakar, Senegal.

The handbook has been translated into French and Portuguese.

In 2005 – 2007, ANECCA partnered with the Institute of Public Health and Development (ISPED), Bordeaux – France, to initiate and conduct a multi-centre, multi-country collaborative research program that described paediatric HIV program set up characteristics in 26 paediatric HIV treatment centres in 17 countries on the African continent. The study also described paediatric HIV treatment outcomes in these centres during the initial stages of introduction of paediatric ART practice in Africa (2004 – 2005). Two papers were published in peer-reviewed journals.